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991.
Mahdi Golshan Hamid R. Habibi Sayyed Mohammad Hadi Alavi 《Fish physiology and biochemistry》2016,42(4):1157-1165
Vinclozolin (VZ) is a pesticide that acts as an anti-androgen to impair reproduction in mammals. However, VZ-induced disruption of reproduction is largely unknown in fish. In the present study, we have established a combination exposure in which adult goldfish were exposed to VZ (30 and 100 μg/L), anti-androgen flutamide (Flu, 300 μg/L), and androgen testosterone (T, 1 μg/L) to better understand effects of VZ on reproductive endocrine system. mRNA levels of kisspeptin (kiss-1 and kiss-2) and its receptor (gpr54), salmon gonadotropin-releasing hormone (gnrh3) and androgen receptor (ar) in the mid-brain, and luteinizing hormone receptor (lhr) in the testis were analyzed and compared with those of control following 10 days of exposure. kiss-1 mRNA level was increased in goldfish exposed to 100 µg/L VZ and to Flu, while kiss-2 mRNA level was increased following exposure to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. gpr54 mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu and 100 µg/L VZ with T. gnrh3 mRNA level was increased in goldfish exposed to 100 µg/L VZ, to Flu, and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. The mid-brain ar mRNA level was increased in goldfish exposed to Flu and to combinations of 30 µg/L VZ with Flu, 100 µg/L VZ with T, and Flu with T. Testicular lhr mRNA level was increased in goldfish exposed to Flu and to combination of 30 µg/L VZ with Flu. These results suggest that VZ and Flu are capable of interfering with kisspeptin and GnRH systems to alter pituitary and testicular horonal functions in adult goldfish and the brain ar mediates VZ-induced disruption of androgen production. 相似文献
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Saiwan Rezaee Hamid Farahmand Mohammad Ali Nematollahi 《Aquaculture International》2016,24(2):479-489
Genetic diversity is vital for the maintenance of genetic pool in cultured shrimps. In order to estimate the current status of genetic diversity in Litopenaeus vannamei shrimp in Iran, as an exotic species, a total of 45 individuals from Amiri and Gorgeaj farms in Jask port of Hormozgan province and one hatchery in Gomishan city of Golestan province, were detected using four microsatellite loci. The number of alleles per locus was 5–10, and the mean effective number of alleles (N e) across populations and loci ranged from 4.834 to 5.148. The overall mean observed heterozygosity (H o) ranged from 0.450 to 0.479, which was lower than the expected one (0.789–0.794). There was nothing remarkable about any of the allele frequencies across populations or loci. The mean inbreeding coefficient (F IS) and pairwise genetic differentiation (F ST) among populations were 41.6 % and 0.133, respectively. The three studied populations departed from Hardy–Weinberg equilibrium (HWE). Analysis of molecular variance revealed 14 % variability among and 86 % within populations. However, considering departing from HWE and the high F IS and F, the moderate pairwise F ST values, importance of introducing genetically diverse broodstock and monitoring to control inbreeding is discussed. 相似文献
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Amir Hossein Saeidnejad Mohammad Kafi Mohammad Pessarakli 《Communications in Soil Science and Plant Analysis》2016,47(8):1048-1057
In order to evaluate the combined effects of salinity stress and Zinc (Zn) applications on wheat growth, an experiment was conducted with the treatments included four Zn levels (0, 10, 20, and 40 mg/kg of dried soil) and three levels of sodium chloride (NaCl) salinity of irrigation water (0, 100, and 200 mM NaCl). Salinity caused a significant reduction in chlorophyll a and b content. The activity of catalase (CAT), superoxide dismutase (SOD), and glutathione reductase (GR) was enhanced as salinity level was increased. The significant enhancement in concentration of sodium due to the salinity was alleviated by Zn addition. Potassium content was increased by Zn treatments. A substantial increase was observed in leaf Zn concentration as the applied level of Zn was increased. Overall, these results indicate some positive and negative interactions of salinity and Zn application, which could be helpful in management of the saline soil and water. 相似文献
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Jinwen Huang Reza Keshavarz Afshar 《Communications in Soil Science and Plant Analysis》2016,47(21):2458-2464
Lentils (Lens culinaris L.) are an important component of the dryland farming systems in the western USA. Optimum nitrogen (N) management can enhance yield and quality of lentils. We conducted a field (at two locations, one with previous history of lentil and the other one without lentil history) and a greenhouse study to evaluate response of lentil to the application of rhizobium inoculant and starter N (control, 22 kg N ha?1 in the form of urea [U], 22 kg N ha?1 in the form of slow-release or environmentally safe nitrogen [ESN], and 22 kg N ha?1 U + 22 kg N ha?1 ESN). In both, the field and the laboratory studies, lentil yield did not respond positively to the experimental treatments. Lentil average yield was 1216 and 1420 kg ha?1 at the field condition. In this rain-fed system, lentil yield was mainly limited by moisture availability, and the application of an external N did not contribute to the yield enhancement. Both of these treatments, however, increased protein content. Compared to the control, the application of rhizobium plus U and ESN enhanced protein content by about 34% (from 23.1 to 30.9%). The application of U+ESN also considerably increased postharvest residual nitrate (NO3)-N in the soil, which can be easily leached and creates environmental pollution. Briefly, the application of U+ESN increases lentil protein content, but more efforts are needed to optimize N management in lentils in order to reduce the environmental concerns in the shallow soil. 相似文献
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